Identification, characterization, and nucleotide sequence of the F17-G gene, which determines receptor binding of Escherichia coli F17 fimbriae

Identification and detection of three new F17 fimbrial variants in Escherichia coli strains isolated from cattle

F17 fimbriae are produced by pathogenic Escherichia coli involved in diarrhea and septicemia outbreaks in calves and lambs. These proteins result from the expression of four different clustered genes, namely f17A, f17D, f17C and f17G, encoding a pilin protein, a periplasmic protein, an anchor protein and an adhesin protein, respectively. Several variants of f17A and f17G genes have been reporte...

Confirmed nucleotide sequence of fanF of Escherichia coli K99 fimbriae.

Nucleotide sequence of the F41 fimbriae subunit gene in Escherichia coli B41.

A clone encoding the F41 f imbnal sub-unit gene from E coli B41, was isolated from an B1BL3 library using an oligonucleotide based on the published N-termlnal amino acid sequence (1). The DNA sequence of an t I66bp fragment revealed an open reading frame encoding a protein of 277 amino acids including a 22 amino acid leader sequence (dash underlined). This corresponds to a mature protein of 290...

The nucleotide sequence of the genes, fanE and fanF of Escherichia coli K99 fimbriae.

K99 fimbriae of enterotoxigenic Escherichia coli consist of eight different subunits. A major subunit called fimbrillin forms fimbrial structure and a minor subunit called adhesin localizes at the tip of fimbriae and recognizes host receptor ganglioside. Within this eight gene cluster, fanE and fanF have not yet been sequenced. In this study, fanE and fanF genes were sequenced by analyzing seve...

Nucleotide sequence of Escherichia coli katE, which encodes catalase HPII.

A 3,466-bp nucleotide sequence containing the katE gene of Escherichia coli has been determined. An open reading frame of 2,259 bp was found and was preceded by a potential ribosome-binding site. The predicted N-terminal sequence agreed with the sequence determined by direct amino acid sequencing, and the predicted direction of transcription was confirmed by expression of the gene cloned in bot...